• Home >
  • Past Seminars

Past Seminars

Here is the list of our past seminars:


Emmanuel Beaurepaire (Lab for optics and biosciences, Ecole Polytechnique). Biophysics Seminar ESPCI-ENS - Emmanuel Beaurepaire (LOB, Ecole Polytechnique)

Friday 9 February 2018 from 13:00 to 14:00 - Amphi Urbain, Ground Floor, Staircase N

Nonlinear optical microscopy of developing and brain tissue

Modern issues in developmental biology and in neuroscience often require tissue-scale measurements of multiple cell parameters. Multiphoton fluorescence microscopy is a key technique for tissue studies with its ability to provide subcellular resolution in thick/live samples. However in practice, current methods are still limited in terms of their imaging speed, accessible depth, non-toxicity, and ability to probe multiple parameters.
We will present some ongoing developments aiming at addressing the issues mentioned above: efficient combination of fluorescence with harmonics contrasts [1,2], multicolor two-photon excitation and its application to 'brainbow'-labelled tissues [3,4], parallelization through light-sheet excitation [5,6], three-photon excitation [7]. We will discuss the application of these advances in developmental biology (embryo and brain development) and in biomedical studies.

Some references:
[1] Olivier, Science (2010). [2] Genthial, Sci Rep (2017). [3] Mahou, Nat Methods (2012). [4] Stringari, Sci Rep (2017). [5] Mahou, Nat Methods (2014). [6] Wolf, Nat Methods (2015). [7] Guesmi, Abdeladim, Light Sci App (in press).






Séminaires récents  (0)


Emmanuel Beaurepaire (Lab for optics and biosciences, Ecole Polytechnique). Biophysics Seminar ESPCI-ENS - Emmanuel Beaurepaire (LOB, Ecole Polytechnique)

Friday 9 February 2018 from 13:00 to 14:00 - Amphi Urbain, Ground Floor, Staircase N

Nonlinear optical microscopy of developing and brain tissue

Modern issues in developmental biology and in neuroscience often require tissue-scale measurements of multiple cell parameters. Multiphoton fluorescence microscopy is a key technique for tissue studies with its ability to provide subcellular resolution in thick/live samples. However in practice, current methods are still limited in terms of their imaging speed, accessible depth, non-toxicity, and ability to probe multiple parameters.
We will present some ongoing developments aiming at addressing the issues mentioned above: efficient combination of fluorescence with harmonics contrasts [1,2], multicolor two-photon excitation and its application to 'brainbow'-labelled tissues [3,4], parallelization through light-sheet excitation [5,6], three-photon excitation [7]. We will discuss the application of these advances in developmental biology (embryo and brain development) and in biomedical studies.

Some references:
[1] Olivier, Science (2010). [2] Genthial, Sci Rep (2017). [3] Mahou, Nat Methods (2012). [4] Stringari, Sci Rep (2017). [5] Mahou, Nat Methods (2014). [6] Wolf, Nat Methods (2015). [7] Guesmi, Abdeladim, Light Sci App (in press).






Archives des anciens séminaires  (219)


Top



See also...

Unregister our newsletter

Gravida potentié lobortïs odio pharetra diam dui nullä leçtus fames malesuada pésuéré, ut quam egestas égét à nunc mi ïpsum pulvinar mauris id, vel (...) 

> More...

Introduction

This weekly seminar aims at gathering researcher form different thematics (physicists, biologists and chemists) and from different institutes in (...) 

> More...